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What is an immunoassay?

David A. Fishman, MD
Gynecologic Oncology
An immunoassay is a test that qualitatively or quantitatively determines the presence of a substance (e.g., a protein) that is caused to react as an antibody or an antigen. An enzyme-linked immunosorbent assay (ELISA) is an example of an immunoassay. If an enzyme is used as a label and attached to the antibody or antigen, the test is then referred to as an enzyme immunoassay. Multiplex bead-based immunoassays offer the capability of concurrently determining the concentrations of antibodies to many different antigens.

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