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Gary Wenk, Psychology, answeredThere are numerous ways to prepare coffee, each produces quite different levels of caffeine per average cup. Generally, the minimum dose a human can detect is about 85milligrams of caffeine. One study actually measured the content of caffeine in a 16 oz cup of coffee prepared at a very popular coffee house. The average dose was 188 mg and the range was huge: from 58 to 564 mg of caffeine across six consecutive days. Thus, it is probably impossible to be sure how much caffeine is in your cup of coffee on any particular day. Therefore, drink it slowly and just wait to see what your brain tells you.